Used in research and diagnostic applications to detect histone H3, particularly in chromatin immunoprecipitation (ChIP) assays, immunofluorescence, and Western blotting. Helps study epigenetic modifications, gene expression regulation, and nuclear structure. Valuable in cancer research for analyzing histone modifications linked to tumor progression. Also employed in autoimmune disease studies where anti-histone antibodies are biomarkers. Its specificity supports accurate detection of histone H3 in complex biological samples.