DL-1,4-Dithiothreitol is widely used in biochemistry and molecular biology as a reducing agent to break disulfide bonds within and between proteins. This helps in maintaining the reduced state of thiol groups, which is crucial for protein structure and function studies. It is also employed in electrophoresis to reduce protein samples, ensuring they are in a denatured state for accurate analysis. Additionally, it plays a role in nucleic acid research by stabilizing RNA and preventing its degradation during extraction and handling. In cell culture, it is used to maintain the redox environment, promoting cell viability and function. Its ability to reduce oxidative stress makes it valuable in various experimental protocols and industrial applications.