TCEP Solution is widely used as a reducing agent in biochemical and molecular biology applications. It effectively breaks disulfide bonds in proteins, helping to denature and solubilize them for analysis, especially in sample preparation for SDS-PAGE and mass spectrometry. Unlike older agents like DTT, TCEP is more stable, odorless, and effective over a broader pH range. It is commonly used in protein purification, peptide mapping, and proteomics workflows. TCEP also prevents re-oxidation of thiol groups during conjugation reactions, making it valuable in bioconjugation and labeling procedures. Its water solubility and compatibility with many buffer systems allow for direct use in various assays without interference.