Used primarily as a redox indicator in biochemical assays, it helps detect the presence of dehydrogenase enzymes by changing color upon reduction. It is widely applied in cell viability tests, where it indicates metabolic activity by forming a blue formazan dye. Additionally, it is employed in histochemistry to localize enzyme activity in tissue samples, aiding in the study of cellular processes. Its ability to visualize oxidative stress makes it valuable in research related to reactive oxygen species and antioxidant activity.