Indophenol Blue is widely used as a redox indicator in various chemical and biochemical assays. It is particularly employed in titrations to detect the endpoint of redox reactions, where it changes color based on the oxidation state of the solution. In analytical chemistry, it serves as a key component in the indophenol method for quantifying ammonia levels in water and biological samples. Additionally, it is utilized in enzymatic assays to monitor dehydrogenase activity, providing a visual or spectrophotometric means to track reaction progress. Its sensitivity to redox conditions makes it a valuable tool in research and industrial applications for analyzing oxidative and reductive processes.