Safranine T is widely used as a biological stain in microscopy to highlight cell structures, particularly in histology and cytology. It is effective in staining nuclei, cartilage, and mucin, making it valuable for differentiating tissues under a microscope. In addition to its role in biological staining, it is employed as a redox indicator in analytical chemistry, where it helps determine the endpoint in titration experiments. Safranine T also finds use in the textile industry as a dye for fabrics, providing vibrant shades of red and pink. Furthermore, it is utilized in photodynamic therapy research due to its photosensitizing properties, which can be harnessed for targeted cell damage in medical treatments.