α-Actinin from chicken gizzard is widely used in research and diagnostic applications due to its role in cytoskeletal organization and muscle contraction. It is employed in studies focused on understanding the structure and function of actin filaments, particularly in muscle and non-muscle cells. Researchers utilize it to investigate cell motility, adhesion, and the mechanics of the cytoskeleton. Additionally, it serves as a critical component in assays designed to study protein-protein interactions, especially those involving actin and other cytoskeletal proteins. In diagnostics, α-Actinin is used as a biomarker in certain muscle-related disorders and autoimmune diseases, aiding in the identification and characterization of these conditions. Its application extends to the development of therapeutic strategies targeting cytoskeletal abnormalities in various diseases.