The Rhesus DKK1 (N256Q) variant with mFc tag is used in research to study Wnt signaling pathway modulation, particularly in bone metabolism and cancer development. The N256Q mutation alters a potential glycosylation site, potentially affecting protein function and stability. It helps in understanding autoimmune diseases and regenerative medicine by regulating stem cell differentiation. Employed in immunoassays and protein interaction studies due to the mFc tag, enabling easy detection and purification. Supports development of therapeutic antibodies and diagnostic tools targeting DKK1-related pathologies.