Mouse SFRP4, His Tag is primarily used in biological research to study the Wnt signaling pathway, which plays a critical role in embryonic development, cell proliferation, and tissue homeostasis. This protein functions as a modulator of Wnt signaling by binding directly to Wnt ligands or to Frizzled receptors, thereby inhibiting pathway activation. Researchers use the His-tagged version for easy detection and purification in assays involving protein-protein interactions, receptor binding studies, and signal transduction analysis.

It is commonly applied in cell culture experiments to investigate the effects of Wnt pathway inhibition on stem cell differentiation, cancer progression, and tissue regeneration. Due to its role in regulating cell fate, Mouse SFRP4, His Tag is also used in studies related to metabolic diseases, bone formation, and eye development. The His tag facilitates immobilization on nickel-coated plates or columns, making it suitable for ELISA, pull-down assays, and surface plasmon resonance (SPR) to quantify binding kinetics. Its recombinant form allows consistent and reproducible results in in vitro and in vivo models.