Used in immunological research to study immune responses against Monkeypox virus, particularly in the development and evaluation of vaccines and therapeutic antibodies. The A30L protein plays a role in viral membrane formation, making it a target for neutralizing antibodies. The mFc tag allows for easy detection and purification in assay systems, supporting studies on antigen-antibody interactions and immune system activation. Commonly applied in ELISA, Western blot, and flow cytometry to assess antibody binding and immune response in preclinical models.