This recombinant protein is primarily used in research and development for immunological studies related to the Monkeypox virus. It serves as an antigen in assays to detect immune responses, aiding in the development and evaluation of vaccines and diagnostic tools. Due to the presence of the His Tag, it can be easily purified and detected in experimental settings, making it valuable in protein interaction studies and antibody production. It is also used in structural studies to understand viral protein function and in screening potential antiviral compounds.