Used in immunological research to study T-cell activation and co-stimulatory signaling pathways. Supports the development of immunotherapies by serving as a ligand in receptor-ligand interaction studies involving CD27. Employed in ELISA, surface plasmon resonance (SPR), and protein-protein interaction assays to quantify binding affinity and evaluate monoclonal antibodies. Facilitates in vitro expansion of T cells and memory T cell studies. The His tag allows for easy purification and detection, making it suitable for recombinant protein workflows in drug discovery and diagnostic applications.