Used in immunological research to study T-cell and B-cell signaling pathways, particularly in the context of immune activation and regulation. Plays a role in the development of therapeutic antibodies and biologics targeting CD30-positive lymphomas, such as Hodgkin's lymphoma and anaplastic large cell lymphoma. The His tag facilitates purification and detection in recombinant protein assays, making it valuable for in vitro binding studies and drug screening. Also employed in ELISA and surface plasmon resonance (SPR) to analyze interactions with ligands or potential inhibitors.