Used in immunological research to study T cell activation and co-stimulatory signaling pathways. Serves as a detection or purification tool in assays involving immune checkpoint interactions. Commonly applied in ELISA, flow cytometry, and protein-protein interaction studies due to the Fc tag enabling easy detection and immobilization. Supports development of therapeutic antibodies targeting 4-1BB for cancer immunotherapy by acting as a binding partner or target antigen in screening and characterization workflows.