Used in research to study inflammatory and immune responses, particularly in diseases like rheumatoid arthritis, Crohn's disease, and other autoimmune conditions. Helps in understanding TNF signaling pathways and developing biologic drugs that target TNF receptors. Commonly employed in protein-protein interaction studies, immunoassays, and as a control in receptor-ligand binding experiments. The His tag facilitates easy purification and detection, making it valuable in recombinant protein workflows.