Used in research to study inflammatory and immune responses by modulating TNF signaling pathways. Binds to TNF-alpha and helps neutralize its activity, making it valuable in developing therapies for autoimmune diseases such as rheumatoid arthritis, Crohn's disease, and psoriasis. The Fc tag enhances solubility and stability, and allows for easy detection and purification in experimental settings. Commonly used in ELISA (including as a standard for measuring TNF-α levels in biological samples like plasma or serum), protein-protein interaction studies, and as a blocking agent in cell-based assays.