Used in immunological research to study T-cell regulation and immune checkpoint pathways. Facilitates the development of therapeutic antibodies and diagnostic assays targeting TIM-3, which plays a key role in suppressing T-cell responses and maintaining immune tolerance. Commonly employed in ELISA, flow cytometry, and protein-protein interaction studies to evaluate binding partners and inhibitor molecules. The His tag enables efficient purification and detection, making it valuable for in vitro and in vivo functional studies related to autoimmune diseases, cancer immunotherapy, and chronic infections.