Used in immunological research to study eosinophil and mast cell regulation, particularly in allergic diseases and asthma. The His tag enables easy purification and detection in protein assays. Commonly applied in binding studies to identify ligands or inhibitors targeting Siglec-8 pathways. Supports development of biologics and therapeutic antibodies for inflammatory conditions. Employed in ELISA, Western blotting, and surface plasmon resonance (SPR) due to its high specificity and affinity when immobilized.