Used in immunological research to study signal regulatory protein alpha (SIRPα)-mediated signaling pathways, particularly in immune cell regulation and macrophage activation. Commonly employed in protein-protein interaction assays, such as binding studies with CD47, to investigate immune checkpoint mechanisms. The His tag facilitates easy purification and detection, making it suitable for in vitro assays, ELISA, surface plasmon resonance (SPR), and flow cytometry applications. Also utilized in therapeutic development targeting the SIRPα-CD47 axis for cancer immunotherapy and autoimmune diseases.