Used in immunological research to study cell signaling pathways involving SIRPγ and its interaction with CD47, particularly in immune cell regulation. Facilitates binding assays and protein-protein interaction studies. Commonly employed in ELISA, surface plasmon resonance, and flow cytometry to analyze ligand-receptor dynamics. The His tag allows for easy purification and detection, making it suitable for in vitro studies focused on immune checkpoint mechanisms and potential therapeutic development.