Used in immunological research to study immune modulation, particularly in macrophage and dendritic cell functions. Serves as a binding partner in assays investigating Siglec-15-mediated suppression of T-cell activation. Employed in protein-protein interaction studies, ELISA, and surface plasmon resonance (SPR) due to its specific recognition properties. Applied in the development of therapeutic antibodies targeting osteoclast-mediated bone resorption and tumor-associated macrophages in cancer immunotherapy. The His tag facilitates easy purification and detection in recombinant expression systems.