Used in immunological research to study signal transduction in immune cells, particularly in interactions involving myeloid cells and macrophages. Plays a role in understanding immune checkpoint pathways by modulating binding to CD47, which influences phagocytosis and immune evasion. Commonly employed in protein-protein interaction assays, ELISA, and flow cytometry to detect binding activity and screen therapeutic agents targeting the CD47-SIRP axis. Also utilized in the development of biologics and antibodies for cancer immunotherapy due to its regulatory function in innate immunity.