Used in research to study axon guidance and neural development due to its role in the Slit-Robo signaling pathway. Commonly employed in protein-protein interaction studies, such as binding assays with Slit ligands. The Fc tag enables easy detection and purification, and supports use in ELISA, flow cytometry, and immunoprecipitation. Also utilized in cancer research, as ROBO1 is involved in tumor suppression and angiogenesis regulation.