Used in neuroscience research to study axon guidance and neural regeneration. Binds to specific receptors on neuronal cells, helping regulate signaling pathways involved in central nervous system development. Employed in protein-protein interaction studies due to its fusion with the Fc tag, enabling easy detection and purification. Applied in immunoassays and surface plasmon resonance to analyze binding kinetics. Also utilized in therapeutic development for spinal cord injury and neurodegenerative diseases by modulating repulsive guidance mechanisms.