Used in immunological research to study the PD-L2/PD-1 signaling pathway, which plays a critical role in immune checkpoint regulation. Commonly employed in binding assays to assess interactions with PD-1 and other receptors, aiding in the development of cancer immunotherapies. Serves as a detection tool in ELISA, flow cytometry, and surface plasmon resonance due to its Fc tag, which facilitates immobilization and detection. Also used in functional studies to modulate T-cell activation and investigate immune evasion mechanisms in tumor microenvironments.