Used in immunological research to study adenosine-mediated immune suppression, particularly in tumor microenvironments. Serves as a binding target for therapeutic antibodies and checkpoint inhibitors in cancer immunotherapy. Applied in ELISA and flow cytometry assays to detect CD73 expression on immune cells. Supports drug development by enabling in vitro studies of ecto-5'-nucleotidase activity and its role in purinergic signaling. Also used in protein-protein interaction studies involving adenosine receptors and extracellular ATP metabolism.