Used in immunological research to study adenosine production and its role in immune regulation. Facilitates assays measuring ecto-5'-nucleotidase activity in tumor microenvironments and inflammatory conditions. Employed in protein-protein interaction studies due to its cell surface expression mimicry when purified with His tag. Supports development of cancer immunotherapies by serving as a target antigen for monoclonal antibody screening. Applied in ELISA, surface plasmon resonance, and flow cytometry to evaluate binding kinetics with therapeutic antibodies or small molecule inhibitors. Enables investigation of purinergic signaling pathways involved in T cell suppression and immune tolerance.