Used as a research reagent in cancer biology, particularly in studies related to targeted therapies for urothelial and breast cancers. Plays a role in the development and validation of antibody-drug conjugates, such as enfortumab vedotin, where Nectin-4 serves as the target antigen. The His tag facilitates purification and detection in immunoassays and binding studies. Commonly employed in protein-protein interaction assays, ELISA, and flow cytometry to evaluate therapeutic antibody binding and specificity. Also utilized in structural studies to understand the molecular basis of cell adhesion and signaling in tumor progression.