Used in cancer research and immunotherapy development due to its role as a tumor differentiation antigen. Serves as a biomarker for malignant mesothelioma and ovarian cancer. Employed in the development of targeted therapies such as CAR-T cells and monoclonal antibodies that bind specifically to mesothelin-expressing tumor cells. The His tag facilitates purification and detection in experimental assays, making it valuable in vaccine studies and diagnostic applications.