Used in immunological research to study the role of Macrophage Migration Inhibitory Factor (MIF) in inflammation, autoimmune diseases, and cancer. The His tag allows for easy purification and detection, making it valuable in protein-protein interaction studies, immunoassays, and as a standard in Western blotting or ELISA. Frequently employed in drug development to screen inhibitors targeting MIF activity, especially in conditions like rheumatoid arthritis, sepsis, and atherosclerosis. Also utilized in cellular signaling studies to understand MIF-mediated pathways involving immune cell activation and cytokine regulation.