Used in immunological research to study cell adhesion mechanisms, particularly in gut-specific lymphocyte trafficking. Plays a role in assays involving integrin binding, such as α4β7, to evaluate inflammatory bowel disease pathways. Commonly applied in ELISA, surface plasmon resonance, and flow cytometry to analyze protein-protein interactions. The His tag facilitates easy purification and detection, making it suitable for recombinant expression systems and therapeutic development targeting mucosal immunity.