Used as a biomarker for hepatocellular carcinoma (HCC), this protein plays a key role in cancer diagnostics and targeted therapy development. It serves as a target antigen in immunoassay development, including ELISA and flow cytometry, to detect anti-GPC3 antibodies or immune responses. Due to its overexpression in liver cancer and other solid tumors like melanoma and squamous cell carcinoma, it supports research in monoclonal antibody therapies and CAR-T cell development. The His tag facilitates easy purification and detection, making it valuable in recombinant protein studies and drug screening platforms.