Human GPNMB with a His Tag is primarily used in biomedical research and therapeutic development. It serves as a key target antigen in immunoassay development, including ELISA and Western blot, due to the His Tag enabling easy detection and purification. Recombinant GPNMB is widely applied in cancer research, particularly in melanoma and glioblastoma, where it is overexpressed and involved in tumor progression and immune modulation. It is also studied in inflammatory diseases and neurodegenerative disorders such as Alzheimer’s disease, where GPNMB plays a role in regulating microglial activity and neuroinflammation. Additionally, His-tagged GPNMB is used in antibody screening and validation for drug development, especially in evaluating monoclonal antibodies targeting GPNMB for use in antibody-drug conjugates (ADCs). Its soluble, purified form supports structural and functional studies to understand ligand-receptor interactions and downstream signaling pathways.