Used in immunological and molecular biology research to study red blood cell membrane proteins, including their role in cell adhesion, immune response, and as receptors for pathogens such as Plasmodium falciparum in malaria infection. Serves as a capture antigen in ELISA, flow cytometry, and Western blot for detecting anti-glycophorin antibodies. Employed in the development of vaccines and validation of monoclonal antibodies targeting CD235a for diagnostic applications. Supports studies on erythrocyte disorders, including hemolytic anemias and blood group antigen variations, as well as protein-protein interactions. The His tag facilitates easy purification via chromatography and detection in recombinant protein assays.