Used in research and development for studying Notch signaling pathways, particularly in the context of neuroendocrine cancers such as small cell lung cancer (SCLC) and other high-grade neuroendocrine neoplasms. The His tag enables easy purification and detection, making it valuable in protein-protein interaction studies, immunoassay development, and as a target for therapeutic antibody screening. Plays a key role in the development of targeted therapies, including antibody-drug conjugates (ADCs) and T-cell engaging bispecific antibodies, due to its selective expression on tumor cells and limited presence in normal tissues.