Used in cancer research, particularly in studying tumor angiogenesis—the formation of new blood vessels that support tumor growth. Plays a role in identifying and targeting endothelial cells in solid tumors, making it valuable for developing monoclonal antibodies and immunotherapies. The His tag allows for easy purification and detection in recombinant protein applications. Commonly used in ELISA, Western blotting, and protein-protein interaction studies to explore its binding partners and signaling pathways in tumor microenvironments.