Used in research to study endothelial cell adhesion and vascular barrier function. Plays a key role in angiogenesis and vascular development studies. Commonly applied in protein-protein interaction assays, including ELISA and surface plasmon resonance, due to the His tag facilitating purification and detection. Supports monoclonal antibody development and validation for targeting vascular endothelial junctions. Employed in cell culture models to investigate endothelial permeability, inflammation, and tumor metastasis.