Used in immunological research to study T-cell activation and immune regulation. Binds to MHC class II molecules and HIV envelope glycoproteins, making it valuable in HIV entry inhibition studies. Employed in protein-protein interaction assays, such as ELISA and surface plasmon resonance, due to the Fc tag enabling easy detection and immobilization. The Fc portion allows for purification via protein A/G affinity chromatography and can mediate effector functions in cellular assays. Commonly used as a capture or detection reagent in immunoassays targeting immune checkpoint pathways.