Used in immunological research to study the role of butyrophilin subfamily 1 member A1 in T-cell regulation and lipid secretion in mammary glands. The His tag allows for easy purification and detection in recombinant protein assays. Commonly applied in ELISA, Western blotting, and protein-protein interaction studies to investigate immune responses and breast milk fat formation. Also utilized in developing antibodies and diagnostic tools targeting butyrophilin-related pathways.