Used in stem cell research to induce differentiation of embryonic stem cells into specific lineages, particularly in the development of mesoderm and trophoblast tissues. Plays a key role in bone and cartilage tissue engineering by promoting osteoblastic differentiation in regenerative medicine applications. Employed in developmental biology studies to understand embryogenesis and morphogen gradient effects. The His Tag facilitates easy purification and detection in experimental systems, making it valuable for in vitro and in vivo signaling studies involving the TGF-β superfamily pathway.