Used in immunological research to study cell adhesion and signaling processes, particularly in immune cell interactions and cancer progression. Serves as a biomarker in diagnostic assays for various cancers, including melanoma and prostate cancer. Employed in therapeutic development targeting tumor microenvironments and immune cell recruitment. The His Tag enables efficient purification via immobilized metal ion affinity chromatography (IMAC) using nickel columns and facilitates easy detection in recombinant protein applications. Valuable as an antigen for developing specific antibodies or in assay systems such as ELISA and Western blot to assess target protein expression in biological samples, as well as in binding studies involving ALCAM-interacting partners.