Used in immunological research to study trophoblast glycoprotein (TPBG, also known as 5T4) function in non-human primates, particularly Cynomolgus and Rhesus macaques, in models relevant to human disease. TPBG/5T4 is highly expressed in various cancer cells but low in normal tissues, making it an attractive target for the development of vaccines or antibody therapies, especially in cancer immunotherapy and regenerative medicine.

The His Tag enables easy purification via affinity chromatography and detection, making it valuable in protein interaction studies, antibody development, and vaccine research. Commonly used in ELISA, Western blot, and flow cytometry assays to evaluate immune responses and antigen specificity.

Additionally, this protein is used to assess the efficacy of molecules designed to bind the 5T4 target prior to human testing, particularly in preclinical studies with animal models closely resembling humans, such as Cynomolgus or Rhesus macaques, which offer high biological compatibility for more accurate prediction of human outcomes.