Used in immunological research to study immune responses and develop monoclonal antibodies targeting mesothelin-expressing tumors. Serves as a critical antigen for antibody validation and immunoassay development due to its high homology with human mesothelin. Supports vaccine testing and therapeutic evaluation in preclinical models, especially in cancer immunotherapy involving mesothelin-positive cancers such as mesothelioma, ovarian, and pancreatic cancers. The His tag facilitates easy purification and detection in experimental systems, enhancing reproducibility in protein-based assays.