Used in immunological research to study the function and signaling of Glypican-3 (GPC3) in non-human primate models, particularly in liver cancer and developmental disorders. The S495A and S509A mutations stabilize the protein by preventing cleavage, allowing for more accurate analysis of GPC3 interactions and antibody binding. The Fc tag enables easy detection, purification, and potential use in immunoassay development or therapeutic antibody testing in preclinical studies. Commonly applied in ELISA, flow cytometry, and binding affinity assays to evaluate anti-GPC3 drug candidates.