Used in bioconjugation for targeted imaging and radiopharmaceutical applications, DOTA-ADIBO enables stable attachment of metal ions to biomolecules via click chemistry. Its ADIBO group reacts rapidly and selectively with azides under physiological conditions, making it ideal for labeling peptides, antibodies, and nanoparticles. This dual functionality supports the development of diagnostic imaging agents, particularly in PET and SPECT, where precise tumor targeting and high in vivo stability are required. It is also employed in pretargeting strategies to improve contrast and reduce radiation exposure.