N-3-oxo-hexadecanoyl-L-Homoserine lactone is an acyl-homoserine lactone (AHL) autoinducer used in quorum sensing research to study bacterial communication and biofilm formation. It serves as a chemical signal that enables bacteria to coordinate gene expression based on population density. Applied in microbiology to understand virulence factors and control bacterial infections. Utilized in synthetic biology for designing genetic circuits and regulating gene expression. Employed in agriculture to develop strategies for managing plant pathogens and enhancing crop protection. Additionally, it is studied for quorum sensing inhibition to develop novel anti-bacterial therapies that disrupt bacterial communication rather than killing the cells.