5-Fluoroquinoxaline is primarily used in the field of medicinal chemistry as a key intermediate in the synthesis of various pharmaceutical compounds. Its structure is particularly valuable in the development of antimicrobial agents targeting bacterial infections. These agents are widely used to treat a range of bacterial diseases due to their activity. Additionally, 5-Fluoroquinoxaline derivatives are explored for their potential in anticancer research, where they are studied for their ability to inhibit specific enzymes or pathways involved in cancer cell proliferation. Its applications also extend to agrochemical research, where it is utilized in the development of novel pesticides and herbicides, leveraging its chemical properties to enhance efficacy and selectivity.