EvaGreen Express 2X qPCR MasterMix-ROX is widely used in quantitative PCR (qPCR) applications for accurate and sensitive detection of nucleic acids. It is particularly valuable in gene expression analysis, where it enables researchers to measure the abundance of specific RNA or DNA targets in real-time. The inclusion of ROX dye ensures normalization of fluorescence signals, improving data consistency across different wells and instruments. This mastermix is compatible with various qPCR instruments and provides a robust platform for high-throughput screening, mutation detection, and pathogen identification. Its ready-to-use formulation simplifies workflow, reducing preparation time and minimizing potential errors. Additionally, it is suitable for applications requiring high specificity, such as SNP genotyping and allelic discrimination.