Used in quantitative real-time PCR (qPCR) to detect and quantify DNA amplification. The premix contains a hot-start Taq DNA polymerase, SYBR Green I dye, dNTPs, and optimized buffer components, enabling specific and sensitive amplification with reduced non-specific products. Ideal for gene expression analysis, pathogen detection, and genotyping. The hot-start feature minimizes enzyme activity at room temperature, improving specificity and yield. SYBR Green dye binds double-stranded DNA, allowing real-time monitoring of PCR product accumulation. Compatible with various real-time PCR instruments and requires only template, primers, and water to run.