2-(Pyrrolidin-3-yl)acetic acid hydrochloride is primarily utilized in pharmaceutical research and development. It serves as a key intermediate in the synthesis of various bioactive compounds, particularly those targeting neurological disorders. Its structure is valuable in designing molecules that interact with neurotransmitter systems, making it useful in the development of drugs for conditions like Parkinson's disease, depression, and anxiety. Additionally, it is employed in the creation of peptidomimetics, which mimic peptides to enhance drug stability and bioavailability. This compound also finds application in organic synthesis, where it acts as a building block for constructing complex molecules with potential therapeutic properties.